Regulatory
Part:BBa_K3245009:Design
Designed by: Jianyi Hu Group: iGEM19_Fudan (2019-10-06)
Enhanced QS promoter regulated by luxR-AHL complex
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 26
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We substituted the -35 to -10 region of the original promoter luxpR-HS with the one of J23100 / J23106, a strong constitutive promoter.
Source
Derivated from the luxpR-HS promoter designed by 2018 Tsinghua team